![]() ![]() ![]() G Remove 1 cm 3 of well-mixed solution from the x10 tube into the x100 tube. SAFETY: Check any relevant hazcards or safety literature for your solvent and the substance under test.Ī Label five appropriate tubes (test tubes or boiling tubes – according to how much solution you are making): 1, x10, x100, x1000, x10000ī Measure 11 cm 3 of your starting solution into the first tube (labelled 1).Ĭ Use a 10 cm 3 syringe or pipette to put 9 cm 3 of solvent (such as distilled water) into each of the other tubes.ĭ Mix the contents of the first test tube thoroughly.Į Remove 1 cm 3 of solution from the first tube, into the tube labelled x10. This allows you to record exactly how much liquid you have dispensed from the pipette. A series of ten-fold dilutions is described as ten-fold serial dilutions. This will depend on the nature of the substance you are diluting and the solvent you are using.ġ Graduated pipettes are numbered as a burette – with liquid reaching the zero mark when the pipette is full. ![]() Read our standard health & safety guidance ![]()
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